KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
L-allo-threonine | pH and temperature not specified in the publication | Escherichia coli | |
10 | - |
L-threonine | pH and temperature not specified in the publication | Escherichia coli | |
14.6 | - |
L-allo-threonine | pH and temperature not specified in the publication | Pseudomonas sp. | |
14.7 | - |
L-threonine | pH and temperature not specified in the publication | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium pasteurianum | - |
- |
- |
Escherichia coli | - |
- |
- |
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. NCIMB10558 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography | Clostridium pasteurianum |
DEAE-Toyopearl column chromatography | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity of 31 units/mg, using L-threonine as substrate, pH and temperature not specified in the publication | Pseudomonas sp. |
additional information | - |
the crude extract has a specific activity of 0.02 units/mg, the purified enzyme has a specific activity of 0.32 units/mg, pH and temperature not specified in the publication | Clostridium pasteurianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-allo-threonine | - |
Escherichia coli | ? | - |
? | |
L-allo-threonine | - |
Pseudomonas sp. | ? | - |
? | |
L-allo-threonine | - |
Pseudomonas sp. NCIMB10558 | ? | - |
? | |
L-threonine | L-TA preferably accepts L-threonine as substrate | Escherichia coli | glycine + acetaldehyde | - |
? | |
L-threonine | L-TA preferably accepts L-threonine as substrate | Pseudomonas aeruginosa | glycine + acetaldehyde | - |
? | |
L-threonine | L-TA preferably accepts L-threonine as substrate | Pseudomonas sp. | glycine + acetaldehyde | - |
? | |
L-threonine | L-TA preferably accepts L-threonine as substrate | Clostridium pasteurianum | glycine + acetaldehyde | - |
? | |
L-threonine | L-TA preferably accepts L-threonine as substrate | Pseudomonas sp. NCIMB10558 | glycine + acetaldehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-TA | - |
Escherichia coli |
L-TA | - |
Pseudomonas aeruginosa |
L-TA | - |
Pseudomonas sp. |
L-TA | - |
Clostridium pasteurianum |
L-threonine aldolase | - |
Escherichia coli |
L-threonine aldolase | - |
Pseudomonas aeruginosa |
L-threonine aldolase | - |
Pseudomonas sp. |
L-threonine aldolase | - |
Clostridium pasteurianum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Escherichia coli | |
pyridoxal 5'-phosphate | dependent on | Pseudomonas aeruginosa | |
pyridoxal 5'-phosphate | dependent on | Pseudomonas sp. | |
pyridoxal 5'-phosphate | dependent on | Clostridium pasteurianum |